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MS2 or MS-2 may refer to:
Science and technology
- Bacteriophage MS2
- Metre per second squared (ms−2), a unit of acceleration
- Lockheed Martin Maritime Systems & Sensors
- Tandem mass spectrometry (MS²)
- MegaSquirt2, a type of MegaSquirt electronic fuel injection controller
- Mighty Servant 2, a heavy-lift ship
- Mississippi Highway 2
- Progress MS-02, a spacecraft
- Southern Railway (U.S.) Class Ms-2, a locomotive
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