TBST

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In molecular biology, TBST (or TTBS) is a mixture of tris-buffered saline (TBS) and Polysorbate 20 (also known as Tween 20). It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays. Tris is a pH buffer that maintains a pH that usually lies around pH=8. Buffered Saline is a salt that provides an isotone solution. Tween is a soap that removes fat droplets from the blot or well.

Contents of TBS-Tween[edit]

The following is a sample recipe for TBST:

Adjust pH with HCl to pH 7.4–7.6

The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.[1]

References[edit]

https://www.gbiosciences.com/Buffers-Reagents-Chemicals/Electrophoresis-Related-Buffers-Chemicals/TBST-10X